Everything about Amorphispironon E
Everything about Amorphispironon E
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Get amorpha fruticosa leaf,, choose by weighing 2kg, be a part of supercritical CO as a result of pulverization method 2In the extractor, as entrainment agent, use is twenty% of a medicinal content pounds with ethanol, extracts, and extraction temperature is fifty ℃, and extracting pressure is 28MPa, CO 2Flow is 3ml/g crude drug/min, plus the extraction time is 4h, and separating nonetheless I temperature is 40 ℃, and tension is 10MPa, and separating nevertheless II temperature is 35 ℃, and force is 7MPa, obtains extract, stirs with ETHYLE ACETATE and tends to make dissolving, filters, plus the filtrating focus drying receives crude extract; Undertake the false indigo spiral shell ketone while in the significant-velocity countercurrent chromatography separation and purification crude extract, its two-phase solvent program is petroleum ether-ethyl acetate-methanol-drinking water, and quantity ratio is 8:3:7:3, on to fill With all the significant velocity adverse current chromatogram put up mutually be stationary period; Rotate main body, pump into down and do moving phase mutually, shifting stage is dissolved crude extract through the sampling valve sample introduction, the UV-detector on-line monitoring; The detection wavelength is 209nm, collects Fake indigo spiral shell ketone element, vacuum concentration; Independent out crystallization, leach crystallization, incorporate the dissolving of 80% methanol eddy; Location recrystallization, filtration, washing are drying to obtain false indigo spiral shell ketone, content material 96.two%. Embodiment four:
Get amorpha fruticosa leaf,, choose by weighing 1kg, be a part of supercritical CO through pulverization course of action 2In the extractor, as entrainment agent, consumption is 18% of the medicinal material fat with ethanol, extracts, and extraction temperature is thirty ℃, and extracting stress is 32MPa, CO 2Flow is 4ml/g crude drug/min, plus the extraction time is three.5h, and separating even now I temperature is thirty ℃, and pressure is 8MPa, and separating however II temperature is 28 ℃, and stress is 7MPa, obtains extract, stirs with ETHYLE ACETATE and would make dissolving, filters, and also the filtrating concentrate drying will get crude extract; Undertake the false indigo spiral shell ketone inside the superior-speed countercurrent chromatography separation and purification crude extract, its two-stage solvent program is petroleum ether-ethyl acetate-methanol-drinking water, and quantity ratio is six:one:five:one, on to fill While using the higher pace adverse latest chromatogram Amorphispironon E post mutually be stationary phase; Rotate principal frame, pump into down and do relocating stage mutually, transferring period is dissolved crude extract via the sampling valve sample introduction, the UV-detector on-line checking; The detection wavelength is 209nm, collects Fake indigo spiral shell ketone element, vacuum concentration; Independent out crystallization, leach crystallization, include the dissolving of 80% methanol eddy; Spot recrystallization, filtration, washing are drying to obtain Fake indigo spiral shell ketone, articles 97.four%. Embodiment three:
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The planning process who the purpose of this invention is to offer a style of Fake indigo spiral shell ketone.
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second plots of ITK binding pocket residues and their interactions with all three chosen compounds within the IMPPAT library and With all the recognised inhibitor. A Withanolide A, B Amorphispironon E, C 27-DHA, and D ITK-inhibitor two
Interactions concerning residues of ITK and the compounds from your IMPPAT library are illustrated as follows: A the positioning of compounds inside Amorphispironone the ITK binding pocket, B an enlarged look at on the interactions, and C a representation in the surface area prospective in the compounds while in the ITK Amorphispironon E binding internet site
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Amorphispironone (Amorphispironon E) is undoubtedly an ichthysanoid isolated from Amorpha fruticosa that exhibits substantial anti-tumor advertising consequences on skin tumors in mice and can be used during the review of tumors.
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